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[19-13C]-Vitamin D3 is a stable isotope-labeled form of Vitamin D3, which is an essential nutrient for maintaining bone health and calcium homeostasis in the body. The isotope labeling of Vitamin D3 involves replacing the 19th carbon atom and all the carbon atoms in the side chain with a stable carbon-13 isotope. This labeling method allows for the detection and quantification of Vitamin D3 in complex biological matrices using advanced mass spectrometry techniques.
The usage of [19-13C]-Vitamin D3 in research and clinical applications lies in its ability to serve as an internal standard for the accurate measurement of Vitamin D3 levels in biological samples. This is especially important in cases where the levels of Vitamin D3 are low or when assessing the efficacy of Vitamin D3 supplementation. It also enables the study of Vitamin D3 metabolism and its role in various physiological processes.
From a chemical perspective, [19-13C]-Vitamin D3 has the same molecular formula as natural Vitamin D3, C27H44O, but with a higher molecular weight due to the stable carbon-13 isotopes. This labeling has no significant impact on the biological activity or functions of Vitamin D3. However, it does enable more precise and sensitive analysis of Vitamin D3 levels in biological samples, making it a valuable tool in clinical research and diagnostic applications.
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