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[1'-13C]adenosine 5'-monophosphate (disodium salt) is a specially labeled variant of the nucleotide AMP (adenosine 5'-monophosphate), which contains a single carbon-13 isotope at the 1' position of the ribose sugar moiety. This labeled compound is commonly used in biochemical and biophysical studies to investigate the structure, function, and dynamics of nucleic acids and their interactions with proteins and other molecules.
The carbon-13 isotope serves as a valuable probe for nuclear magnetic resonance (NMR) spectroscopy, as it gives rise to distinctive signals in the NMR spectrum that can be used to derive information about the conformation and dynamics of the labeled molecule. Additionally, [1'-13C]AMP is often used in studies of RNA and DNA processing, such as RNA splicing and editing, as well as in studies of RNA and DNA folding and stability.
Chemically, [1'-13C]AMP is a disodium salt of the nucleotide, meaning that it consists of the AMP molecule with two sodium ions bound to it. The compound is soluble in water and other polar solvents, and can be readily prepared from commercially available starting materials using standard synthetic methods. Its molecular formula is C10H12N5O7PNa2, and its molecular weight is approximately 419.2 g/mol. Overall, [1'-13C]AMP is a useful and versatile tool for exploring the properties of nucleic acids and their interactions with other biomolecules.
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