(-)-Riboflavin 5-phosphate 13C4, 15N2

[13C4, 15N2]-(-)-Riboflavin 5-phosphate is a modified derivative of riboflavin, also known as vitamin B2. This compound is a coenzyme that plays a vital role in many metabolic pathways, particularly in the production of energy from carbohydrates, fats, and proteins. Riboflavin 5-phosphate is the active form of riboflavin and is involved in a number of enzymatic reactions in the body. The addition of carbon-13 and nitrogen-15 isotopes to the molecule allows for more precise tracking of the compound during metabolic studies. The compound is often used in research studies to investigate the metabolic pathways of riboflavin and to determine the efficacy of various drugs or treatments that impact these pathways. It is also used in medical diagnostics to assess the status of riboflavin levels in the body, as low levels of this vitamin can lead to a variety of health issues, including anemia, skin disorders, and vision problems. Chemically, [13C4, 15N2]-(-)-Riboflavin 5-phosphate is a complex molecule consisting of a riboflavin moiety bound to a phosphate group. The addition of the carbon-13 and nitrogen-15 isotopes to the molecule does not significantly alter its chemical properties, but it does allow for more precise tracking of the molecule during metabolic studies. The compound is typically synthesized using a combination of chemical and enzymatic methods, and is purified through a series of chromatographic techniques before use.