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(1-Oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-methyl) Methanethiosulfonate-d15 is a chemical compound that is widely used in various research fields, including the fields of chemistry, biochemistry, and biophysics. This compound is a deuterated derivative of the commonly used spin label Methanethiosulfonate (MTSL). The deuterium labeling in this compound makes it useful in studying the dynamics and interactions of proteins and other biomolecules using electron paramagnetic resonance (EPR) spectroscopy.
This compound is generally used as a spin label, which is a molecule that is attached to the biomolecule of interest to enable the detection of its structure and dynamics. The spin label is a small molecule that is attached to the biomolecule through a chemical bond, and it is used to probe the local environment of the biomolecule. The EPR spectroscopy technique allows researchers to study the mobility of the spin label, which provides information about the flexibility and dynamics of the underlying biomolecule.
The chemical structure of (1-Oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-methyl) Methanethiosulfonate-d15 consists of a nitroxide radical attached to a thiol-reactive methanethiosulfonate group. The deuterium labeling of this compound is achieved by replacing the hydrogen atoms in the methyl groups of the nitroxide radical with deuterium atoms. This labeling enables researchers to distinguish between different types of motions in the EPR spectra.
In summary, (1-Oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-methyl) Methanethiosulfonate-d15 is a useful spin label for studying the dynamics and interactions of biomolecules using EPR spectroscopy. Its deuterium labeling provides a unique advantage in studying the mobility of the spin label, which is crucial in understanding the flexibility and dynamics of the underlying biomolecule.
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