[13C, 15N2]-Orotic acid hydrate

[13C, 15N2]-Orotic acid hydrate is a stable isotope labeled form of orotic acid, which is a heterocyclic compound that contains both nitrogen and carbon in its structure. It is mainly used in research applications to investigate the metabolism of nucleic acids and amino acids. Specifically, it is used in studies that focus on how the body synthesizes and uses pyrimidine nucleotides, which are building blocks of DNA and RNA. The stable isotopes, 13C and 15N, are incorporated into the orotic acid molecule to allow for tracing and identification of metabolic pathways. These isotopes have different masses than the naturally occurring isotopes of carbon and nitrogen, which allows for easy detection using mass spectrometry. [13C, 15N2]-Orotic acid hydrate is typically administered orally to subjects in research studies. It is rapidly absorbed in the intestine and transported to the liver, where it is metabolized. The isotope-labeled orotic acid is then incorporated into nucleotides and amino acids, which can be detected and measured in various tissues and fluids. Overall, [13C, 15N2]-Orotic acid hydrate is a valuable tool for researchers studying nucleic acid and amino acid metabolism. Its stable isotope labels allow for precise tracking and identification of metabolic pathways, which can lead to a better understanding of how these processes are regulated in health and disease.