[1'-13C]thymidine

[1'-13C]thymidine is a labeled version of thymidine that contains a carbon-13 isotope at the 1' position of the sugar ring. This isotope labeling makes it a useful tool in various research applications, such as studying DNA replication and DNA damage and repair mechanisms. One common use of [1'-13C]thymidine is in metabolic labeling experiments, where cells are grown in the presence of the labeled thymidine. The labeled thymidine is incorporated into newly synthesized DNA, allowing researchers to track the fate of the labeled DNA within the cell. This technique can provide insight into DNA replication dynamics, as well as the turnover rates of different cell populations. In addition to its use in metabolic labeling experiments, [1'-13C]thymidine can also be used in NMR spectroscopy studies. The carbon-13 isotope provides a unique signal in NMR spectra, allowing researchers to study the dynamics and structure of DNA and DNA-protein complexes. From a chemical perspective, [1'-13C]thymidine is a modified nucleoside that is structurally similar to regular thymidine. It is synthesized through the modification of thymidine via enzymatic and chemical methods. The resulting labeled thymidine can be purified and used in a variety of research applications.