[1',2',3',4',5'-13C5;1,3-15N2]thymidine

[1',2',3',4',5'-13C5;1,3-15N2]thymidine is a labeled form of thymidine that is widely used in the field of molecular biology and biochemistry. Thymidine is a nucleoside that is a part of DNA, and the labeled form is used to study the mechanism of DNA replication and repair processes in cells. The chemical formula of [1',2',3',4',5'-13C5;1,3-15N2]thymidine is C14H16N3O5. It has five carbon-13 atoms and two nitrogen-15 atoms that are labeled isotopes. These isotopes allow for the tracking of the labeled thymidine within the cell and can be detected by various analytical techniques, such as mass spectrometry. The labeled thymidine is incorporated into the DNA of the cells during the replication process, which allows for the measurement of DNA synthesis rates. It is also used to study the effects of DNA damage and repair on the replication process. The labeled thymidine can be used in a variety of assays, including cell proliferation assays, DNA labeling assays, and autoradiography. Overall, [1',2',3',4',5'-13C5;1,3-15N2]thymidine is a valuable tool in molecular biology and biochemistry research, allowing for the visualization and measurement of DNA synthesis rates and the study of DNA replication and repair processes.