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[1',2',3',4',5'-13C5]2'-deoxyguanosine monohydrate is a stable isotopic labeled compound that is commonly used in molecular biology research to study the structure and function of DNA. It is a modified form of 2'-deoxyguanosine, a nucleoside that is a building block of DNA, where five carbon atoms in the sugar moiety are replaced with their stable isotope labeled counterparts. This labeling makes it possible to track the flow of carbon atoms in cellular processes and to analyze the structure and dynamics of DNA using various spectroscopic techniques.
The compound is typically used in experiments that involve nucleotide synthesis, DNA replication, and DNA damage and repair. It can be incorporated into newly synthesized DNA strands during PCR amplification or DNA sequencing, allowing researchers to study the kinetics and accuracy of DNA polymerases. Additionally, it can be used in various labeling and tracking experiments, such as metabolic labeling, protein-DNA cross-linking, and pull-down assays.
From a chemical standpoint, [1',2',3',4',5'-13C5]2'-deoxyguanosine monohydrate is a white to off-white powder that is soluble in water, methanol, and ethanol. It has a molecular formula of C10H12N5O4 and a molecular weight of 281.24 g/mol. The compound is stable under normal storage conditions and should be kept in a cool, dry place away from light and moisture. It is important to handle the compound with care and to use appropriate protective equipment, as it can be harmful if ingested or inhaled.
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