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[1',2',3',4',5'-13C5]guanosine monohydrate is a stable isotope-labeled analog of guanosine monohydrate, which is a nucleoside consisting of guanine and ribose. The isotope-labeled guanosine monohydrate is typically used in scientific research as a tracer for metabolic and biochemical studies.
The primary usage of [1',2',3',4',5'-13C5]guanosine monohydrate is in nuclear magnetic resonance (NMR) spectroscopy, which is a powerful technique used to study the structure and dynamics of molecules. The labeled guanosine monohydrate can be incorporated into RNA and DNA molecules, allowing for the analysis of their conformation and interaction with other molecules. It can also be used to study the metabolism of guanosine and related compounds in cells and tissues.
The chemical properties of [1',2',3',4',5'-13C5]guanosine monohydrate are similar to those of its unlabeled counterpart, with the exception of the presence of five carbon-13 isotopes in the ribose moiety. Carbon-13 is a stable, non-radioactive isotope of carbon that is commonly used in NMR spectroscopy. The labeling of guanosine monohydrate with carbon-13 allows for the detection of specific carbon atoms in the molecule, providing valuable information about its structure and interactions.
Overall, [1',2',3',4',5'-13C5]guanosine monohydrate is a valuable tool for researchers studying nucleic acids, metabolism, and other biochemical processes. Its stable isotope labeling allows for precise analysis and interpretation of experimental data, leading to a deeper understanding of these important biological systems.
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