2-(4,6-Diamino-1,3,5-triazin-2-yl)sulfanylethanesulfonic Acid-d4

2-(4,6-Diamino-1,3,5-triazin-2-yl)sulfanylethanesulfonic Acid-d4, also known as DTSSP-d4, is a deuterated form of the chemical compound DTSSP, which is used as a crosslinking reagent in biochemistry and molecular biology experiments. DTSSP-d4 is a water-soluble compound that is used to crosslink proteins, DNA, RNA, and other biomolecules. This is achieved by reacting DTSSP-d4 with the target molecules, which results in the formation of covalent bonds between them. This crosslinking allows for the identification of protein complexes, protein-protein interactions, and protein-DNA interactions, which are essential in understanding biological processes. DTSSP-d4 is also used in the purification of proteins, where it is used to immobilize the target protein on a resin for affinity chromatography. DTSSP-d4 is more stable than DTSSP, making it a better choice for long-term storage and use in experiments. Chemically, DTSSP-d4 is a deuterated form of DTSSP, with four deuterium atoms replacing four hydrogen atoms in the molecule. This substitution changes the mass of the molecule, making it useful in mass spectrometry experiments, where it can be used as a stable isotope-labeled internal standard. In conclusion, DTSSP-d4 is a valuable tool in biochemistry and molecular biology experiments, used for crosslinking and protein purification. Its deuterated form makes it useful in mass spectrometry experiments as well.