2-DEOXYGUANOSINE 5-TRIPHOSPHATE, AMMONIUM SALT 15N5

2-Deoxyguanosine 5-triphosphate, ammonium salt 15N5, also known as dGTP, is a nucleotide triphosphate that is commonly used in molecular biology research. It is a modified form of deoxyguanosine with three phosphate groups attached to the 5' carbon of the deoxyribose sugar, and an ammonium salt added to the nitrogen-15 position of the guanine base. The ammonium salt 15N5 labeling allows for isotope labeling for tracking experiments. This nucleotide triphosphate is used in many applications including DNA sequencing, PCR amplification, and DNA synthesis. It is incorporated into newly synthesized DNA by DNA polymerase during PCR amplification and DNA sequencing, making it an essential component in these techniques. DNA synthesis also requires the use of dGTP, along with the other nucleotide triphosphates, to create new strands of DNA. As a chemical, dGTP is a white to off-white powder that is stable when stored at -20°C. It has a molecular weight of 507.2 g/mol and a melting point of 220-223°C. It is soluble in water and has a pH range of 7-8. In conclusion, 2-Deoxyguanosine 5-triphosphate, ammonium salt 15N5, is a crucial component in many molecular biology techniques, including PCR amplification, DNA sequencing, and DNA synthesis. Its ammonium salt 15N5 labeling allows for isotope labeling for tracking experiments. Its stability and solubility make it a versatile and useful chemical in the laboratory.