3’,5’-Bis-O-(tert-butyldimethylsilyl)thymidine-d3

3’,5’-Bis-O-(tert-butyldimethylsilyl)thymidine-d3 is a synthetic nucleoside that is commonly used in molecular biology and biochemistry research. It is a deoxyribonucleoside that contains a deuterium atom at the 3’ position of the thymidine molecule, making it useful for isotopic labeling experiments. This compound is often used as a building block in the synthesis of oligonucleotides, which are short strands of DNA or RNA that can be used for a variety of applications, such as gene expression analysis, gene editing, and drug discovery. The tert-butyldimethylsilyl (TBDMS) groups at the 3’ and 5’ positions of the thymidine molecule provide protection for the hydroxyl groups, allowing for selective deprotection during the synthesis process. In terms of chemical information, 3’,5’-Bis-O-(tert-butyldimethylsilyl)thymidine-d3 has a molecular formula of C22H37D3N2O5Si2 and a molecular weight of 540.79 g/mol. It is a white to off-white powder with a purity of at least 98% and a melting point of around 150-155°C. Overall, 3’,5’-Bis-O-(tert-butyldimethylsilyl)thymidine-d3 is a valuable tool for researchers working in the fields of molecular biology and biochemistry, allowing for precise isotopic labeling of nucleic acids and facilitating the synthesis of custom oligonucleotides for a wide range of applications.