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[3'-D]guanosine monohydrate is a modified form of guanosine, which is a nucleoside composed of a nitrogenous base called guanine and a five-carbon sugar molecule called ribose. The modification in [3'-D]guanosine monohydrate involves the removal of the hydroxyl group at the 3' position of the ribose sugar molecule, and its replacement with a deuterium atom. This modification makes the molecule less susceptible to enzymatic degradation and enhances its stability, making it suitable for various biochemical and biophysical applications.
[3'-D]guanosine monohydrate is commonly used as a substrate for enzymes that recognize guanosine, such as guanine nucleotide-binding proteins (G proteins) and guanine-specific ribonucleases. It is also used as a precursor for the synthesis of modified nucleotides and nucleic acids, which have potential applications in gene therapy, drug discovery, and nanotechnology.
From a chemical standpoint, [3'-D]guanosine monohydrate is a white crystalline powder that is soluble in water and slightly soluble in ethanol. Its molecular formula is C10H12D1N5O6.H2O, and its molecular weight is 317.23 g/mol. The molecule has a melting point of 202-205°C, and its UV absorption spectrum shows a maximum wavelength of 252 nm. The purity of [3'-D]guanosine monohydrate is typically greater than 98%, and it is stable under normal storage conditions. Overall, [3'-D]guanosine monohydrate is a valuable tool for studying nucleic acid structure and function, and for developing new therapeutic agents.
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