[3-13C]-L-Glutamic acid

[3-13C]-L-Glutamic acid is a stable isotope labeled compound that is used in various research applications such as metabolic labeling, protein structure analysis, and isotope tracing studies. It is a modified form of the amino acid L-glutamic acid, in which three carbon atoms are replaced with carbon-13 isotopes. This modification allows for the identification and quantification of metabolic pathways in complex biological systems. In metabolic labeling studies, [3-13C]-L-Glutamic acid is used to trace the fate of the carbon atoms in the metabolic pathways. It is administered to cell cultures or organisms, where it is incorporated into cellular molecules such as proteins and nucleic acids. The labeled carbon atoms can be traced using mass spectrometry, providing insights into the metabolism of the cells. [3-13C]-L-Glutamic acid is also used in protein structure analysis through nuclear magnetic resonance (NMR) spectroscopy. The labeled carbon atoms provide important information on the conformation and dynamics of the protein molecule, which is useful in drug discovery and protein engineering. The chemical formula of [3-13C]-L-Glutamic acid is C5H7NO4, and its molecular weight is 169.12 g/mol. It is a white crystalline powder that is soluble in water and has a melting point of 200-205°C. It is stable under normal conditions and does not pose any significant health hazards.