2’-Deoxyguanosine-13C,15N2

2’-Deoxyguanosine-13C,15N2 is a stable isotope-labeled form of the nucleoside 2’-deoxyguanosine, which is a component of DNA. The molecule is labeled with Carbon-13 and Nitrogen-15 isotopes, which can be detected using mass spectrometry techniques. This labeled molecule is often used in research studies to investigate DNA replication, repair, and metabolism. One of the main uses of 2’-Deoxyguanosine-13C,15N2 is in metabolic flux analysis, which is a technique used to study the metabolic pathways involved in cellular processes. By using stable isotopes, researchers can track the movement of molecules through different pathways and gain insights into how these processes are regulated. The labeled 2’-Deoxyguanosine-13C,15N2 can be incorporated into DNA during replication, and the labeled atoms can be traced to determine the rate of DNA synthesis and turnover. In addition to metabolic flux analysis, 2’-Deoxyguanosine-13C,15N2 can also be used in studies of DNA damage and repair. By introducing labeled nucleosides into cells, researchers can track the incorporation of damaged nucleotides into DNA and investigate the mechanisms by which cells repair this damage. Overall, 2’-Deoxyguanosine-13C,15N2 is a valuable tool for studying DNA replication, repair, and metabolism. Its stable isotope labeling allows for precise measurements and insights into cellular processes that would be difficult to obtain using other techniques.