5α-Cholestane-2,2,3,3,4,4-d6

5α-Cholestane-2,2,3,3,4,4-d6 is a deuterated analog of 5α-cholestane, a naturally occurring steroid found in animal tissues. It is labeled with six deuterium atoms in specific positions, making it useful for research purposes such as tracer studies and metabolic labeling. 5α-Cholestane-2,2,3,3,4,4-d6 is commonly used as an internal standard in mass spectrometry-based analyses such as gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). Its chemical structure and isotopic labeling allow for accurate determination of the concentration and distribution of endogenous 5α-cholestane in biological samples. In addition, 5α-Cholestane-2,2,3,3,4,4-d6 is also used in the synthesis of other deuterated steroids for research purposes. It can be incorporated as a precursor in the synthesis of deuterated analogs of other steroid hormones such as testosterone and estrogen. The chemical properties of 5α-Cholestane-2,2,3,3,4,4-d6 include a melting point range of 130-134°C and a boiling point of 360-370°C. It is insoluble in water but soluble in organic solvents such as chloroform and ethanol. In conclusion, 5α-Cholestane-2,2,3,3,4,4-d6 is a valuable tool for research in the fields of mass spectrometry and steroid metabolism. Its isotopic labeling and chemical properties make it useful for a variety of applications such as metabolic labeling and synthesis of deuterated steroids.