5β-Cholestan-3β-ol-2,2,3,4,4-d5

5β-Cholestan-3β-ol-2,2,3,4,4-d5, also known as deuterated cholesterol, is a stable isotope-labeled derivative of cholesterol. This compound is used mainly in research and analytical applications, particularly in studies of cholesterol metabolism and biosynthesis. The deuterium labeling in this compound offers several advantages over non-labeled cholesterol, such as improved sensitivity and specificity in mass spectrometry-based analyses. Chemically, 5β-Cholestan-3β-ol-2,2,3,4,4-d5 is a sterol molecule with a hydroxyl functional group (-OH) attached to the third carbon atom of the steroid nucleus. It is structurally similar to cholesterol, which is an essential component of cell membranes and a precursor to many important hormones and bile acids. However, the five deuterium atoms incorporated into this compound make it slightly heavier and more stable than natural cholesterol. In research, 5β-Cholestan-3β-ol-2,2,3,4,4-d5 is used as an internal standard or reference compound for quantifying cholesterol and its metabolites in biological samples. It can also be used to study the metabolic fate of cholesterol in cells and tissues, as well as to investigate the effects of drugs and other compounds on cholesterol metabolism. Overall, the use of deuterated cholesterol is a valuable tool for advancing our understanding of cholesterol biology and its role in health and disease.