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7alpha,12alpha-Dihydroxy-5beta-cholestan-3-one-d7 is a stable isotope-labeled steroid used in biochemical research. It is also known as 7,12-dihydroxy-5-cholesten-3-one-d7. The compound is primarily used as a reference standard in mass spectrometry procedures and analytical chemistry. It has a molecular formula of C27H39D7O3 and a molecular weight of 431.72 g/mol.
The compound is an intermediate in the biosynthesis of bile acids. Bile acids are important for the digestion of fats and the absorption of fat-soluble vitamins. 7alpha,12alpha-Dihydroxy-5beta-cholestan-3-one-d7 is formed from the oxidation of 7alpha-hydroxycholesterol by the enzyme CYP7B1 and is further metabolized to form the bile acid chenodeoxycholic acid.
In addition to its role in bile acid biosynthesis, 7alpha,12alpha-Dihydroxy-5beta-cholestan-3-one-d7 has been studied for its potential as a biomarker for various diseases. For example, elevated levels of this compound have been found in patients with non-alcoholic fatty liver disease, which is a common liver disorder associated with obesity and type 2 diabetes.
Overall, 7alpha,12alpha-Dihydroxy-5beta-cholestan-3-one-d7 is a valuable compound in biochemical research and has potential as a biomarker for disease diagnosis and monitoring. Its stable isotope labeling allows for accurate quantification and identification in mass spectrometry procedures.
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