Crystal Violet-d6 hydrate

Crystal Violet-d6 hydrate is a type of biological stain that is widely used in histology, microbiology, and other related fields. It is a deuterated derivative of Crystal Violet, which is a popular dye for gram-staining of bacteria. The deuterium substitution in Crystal Violet-d6 hydrate provides a unique chemical signature that makes it useful in various applications, particularly in chemical analysis and mass spectrometry. In microbiology, Crystal Violet-d6 hydrate is commonly used to differentiate between gram-positive and gram-negative bacteria. Gram-positive bacteria retain the dye, while gram-negative bacteria do not. This staining technique is crucial in identifying bacterial species and determining appropriate treatment protocols. In histology, Crystal Violet-d6 hydrate is used to stain cell nuclei and other structures to facilitate microscopy analysis. It is also used in tissue culture to identify and quantify cell viability. Chemically, Crystal Violet-d6 hydrate is a triarylmethane dye that contains three aromatic rings and a central carbon atom. The deuterium substitution occurs at the carbon atom, resulting in a slight shift in the dye's spectral properties and mass. The hydrate form of Crystal Violet-d6 is water-soluble and has a purple color. It has a molecular weight of 441.58 g/mol and a melting point of 280°C. In conclusion, Crystal Violet-d6 hydrate is a versatile biological stain that finds use in various applications ranging from microbiology to chemical analysis. Its deuterium substitution provides a unique chemical signature that makes it useful in mass spectrometry and other applications where chemical identification is crucial.