Calcifediol is a widely used pharmaceutical ingredient that is used in the treatment of a variety of medical conditions. As with all pharmaceuticals, calcifediol must be pure and free of impurities to ensure its safety and efficacy. Impurities in calcifediol may arise during the manufacturing process or during storage and handling. These impurities can affect the quality and stability of the drug and may pose a risk to patient safety. Therefore, the identification and quantification of calcifediol impurities is critical for ensuring the safety and efficacy of this important pharmaceutical ingredient.