Moxidectin is a semi-synthetic macrocyclic lactone that is widely used as an anthelmintic in veterinary medicine. The production of Moxidectin involves the use of various chemical reactions, which can lead to the formation of impurities. These impurities can have toxic and harmful effects on the environment and living organisms. Therefore, it is crucial to control the impurities during the manufacturing process to ensure the safety and efficacy of the final product. The identification and quantification of Moxidectin impurities are essential for regulatory compliance and quality control purposes.