Topotecan is an anticancer drug that is used to treat ovarian and lung cancer. However, during the manufacturing process, impurities may be formed as by-products. These impurities can potentially affect the safety and efficacy of the drug. Therefore, it is important to identify and characterize these impurities to ensure that the drug meets the required standards for pharmaceutical use. Various analytical techniques such as HPLC, LC-MS, and NMR spectroscopy are used for the detection and characterization of these impurities.
