Vitexin is a flavonoid compound found in various plants, including passionflower and hawthorn. However, during the production process of vitexin, impurities may be generated due to various factors such as raw material quality, processing techniques, and storage conditions. These impurities can affect the purity and quality of the final product, and therefore, it is essential to identify and quantify them to ensure the safety and efficacy of vitexin-based products.