Guanine-4,5-13C2,7-15N

Guanine-4,5-13C2,7-15N is a stable isotope-labeled compound that is primarily used as a research tool in molecular biology, biochemistry, and pharmacology. It is a modified form of guanine, a naturally occurring nitrogenous base found in DNA and RNA, where two carbon atoms at positions 4 and 5 are replaced with stable isotopes of carbon (13C) and two nitrogen atoms at positions 7 and 15 are replaced with stable isotopes of nitrogen (15N). The stable isotope labeling of guanine-4,5-13C2,7-15N allows for the tracking of the fate of the molecule in living organisms and for the identification of its role in biological processes. It is commonly used as a tracer in metabolic studies, where the labeled guanine is incorporated into various metabolic pathways, allowing for the measurement of metabolic flux rates and the identification of metabolic intermediates. Chemically, guanine-4,5-13C2,7-15N behaves similarly to its natural counterpart, but with some distinct differences. The stable isotopes in the molecule affect its physical and chemical properties, including its mass, vibrational frequencies, and magnetic resonance properties. These differences can be exploited in various analytical techniques, including mass spectrometry, NMR spectroscopy, and vibrational spectroscopy, enhancing the sensitivity and accuracy of these methods. In summary, guanine-4,5-13C2,7-15N is a valuable research tool that is widely used in various fields of science for studying biological processes, metabolic pathways, and disease mechanisms. Its stable isotope labeling provides unique advantages for tracking and identifying the fate of the molecule in living systems and for enhancing analytical methods.