Ascomycin is a macrolide antibiotic used to prevent organ transplant rejection and to treat skin conditions such as eczema and psoriasis. However, the drug can contain impurities that may affect its safety and efficacy. Some of the known impurities in Ascomycin include Ascomycin B, Ascomycin C, Ascomycin D, and Ascomycin E. These impurities can be characterized and quantified through various analytical techniques, ensuring that Ascomycin is safe and effective for use.