Clofarabine is a chemotherapy drug used to treat certain types of leukemia. However, during the manufacturing process, impurities may be generated which can impact the safety and efficacy of the drug. These impurities need to be identified, quantified, and controlled to ensure the final product meets regulatory requirements and is safe for patient use. Analytical methods are used to detect and measure these impurities, and steps are taken to minimize their formation during manufacturing.