Maytansine is a potent cytotoxic agent used in the development of antibody-drug conjugates (ADCs) for cancer treatment. However, the synthesis of maytansine can result in the presence of impurities that can affect the efficacy and safety of the drug. These impurities may include derivatives of maytansine, isomers, and other related compounds. It is important to identify and quantify these impurities to ensure the quality and purity of maytansine for clinical use.